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KMID : 0858620050090010035
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Korean Journal of Audiology
2005 Volume.9 No. 1 p.35 ~ p.42
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Developmental Change of P2Y4 Receptor in Outer Sulcus Cells of the Rat
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Lee Jun Ho
Han Doo-Hee
Heo Jeong-Hwa
Kim Chang-Hee
Chang Sun-O
Kim Chong-Sun
Oh Seung-Ha
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Abstract
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Cochlear outer sulcus cells (OSC) are located between the organ of Corti and K+ secretory cells and known to sustain transepithelial cation currents. It has been thought that P2X2 receptors in OSC provide parasensory K+ efflux pathway in response to intense noise exposure. However, little is known during development. The purpose of this study is to investigate purinergic receptors in developing rat OSC using voltage-sensitive vibrating probe technique. Results showed that there was no response to 100 μM ADP, UDP, and adenosine at any postnatal age. The response to UTP was greatest at postnatal day 1 (P1) and rapidly decreased to near adult level until P7 when the endolymphatic ion composition is known to be accomplished. This UTP-responsive P2Y receptor was found to be P2Y4, evidenced by the inhibition of UTP effect in the presence of 100 μM reactive blue 2, an antagonist at rat P2Y4. These temporal changes of purinergic receptors in developing OSC would be associated with cellular differentiation and/or establishment of the endolymphatic ion composition for normal auditory transduction.
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KEYWORD
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Voltage-sensitive vibrating probe, P2Y receptor, Cochlea, Outer sulcus cell
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